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sirius red fast green collagen staining kit  (Chondrex Inc)


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    Structured Review

    Chondrex Inc sirius red fast green collagen staining kit
    TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using <t>Sirius</t> <t>Red/Fast</t> Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.
    Sirius Red Fast Green Collagen Staining Kit, supplied by Chondrex Inc, used in various techniques. Bioz Stars score: 96/100, based on 297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sirius+red+collagen+staining+kit/Sirius+Red%2FFast+Green+Collagen+Staining+Kit/pmc13120883-103-8-14
    Average 96 stars, based on 297 article reviews
    sirius red fast green collagen staining kit - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Time-restricted feeding improves metabolic flexibility, promotes beiging, and mitigates fibro-inflammation in the adipose tissue of aged mice"

    Article Title: Time-restricted feeding improves metabolic flexibility, promotes beiging, and mitigates fibro-inflammation in the adipose tissue of aged mice

    Journal: The Journals of Gerontology Series A: Biological Sciences and Medical Sciences

    doi: 10.1093/gerona/glag085

    TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using Sirius Red/Fast Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.
    Figure Legend Snippet: TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using Sirius Red/Fast Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.

    Techniques Used: Staining

    Related Articles

    Staining:

    Article Title: The tumor microenvironment shows a hierarchy of cell-cell interactions dominated by fibroblasts.
    Article Snippet: .. Cells were left for 7 days in culture to assure confluence before performing collagen content measurement using a commercial Sirius Red collagen staining kit (Chondrex, Cat.9046), and measured by Cytation 5-Imaging Reader (Biotek). ..

    Article Title: Cancer-associated fibroblast compositions change with breast cancer progression linking the ratio of S100A4 + and PDPN + CAFs to clinical outcome.
    Article Snippet: .. The cells were left for 4 d in culture to assure confluence before preforming collagen content measurement using a commercial Sirius Red collagen staining kit (Chondrex). .. Images were taken with a Leica DMI8 wide-field (inverted) microscope, objective ×10/0.25, using a DFC310FX color camera.

    Article Title: Photoprotective Effects of a Hyperoside-Enriched Fraction Prepared from Houttuynia cordata Thunb. on Ultraviolet B-Induced Skin Aging in Human Fibroblasts through the MAPK Signaling Pathway
    Article Snippet: .. After 48 h of incubation, the cultured medium was kept and the collagen content was determined using a Sirius Red collagen staining kit (Chondrex Inc., Redmond, WA, USA). ..

    Article Title: A cell circuit approach to dissect fibroblast-macrophage interactions in the tumor microenvironment
    Article Snippet: .. Cells were left for 7 days in culture to assure confluence before performing collagen content measurement using a commercial Sirius Red collagen staining kit (Chondrex, Cat.9046), and measured by Cytation 5-Imaging Reader (Biotek). ..

    Article Title: SIRT6 activation attenuates inflammatory-fibrogenic events, improves lung function and survival in experimental pulmonary fibrosis.
    Article Snippet: Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal interstitial lung disease characterized by excessive extracellular-matrix deposition and unalterable lung remodeling.. MDL-800 is a selective small-molecule activator of sirtuin-6 (SIRT6) with emerging anti-inflammatory and antifibrotic potential.. In this study, we evaluated the pharmacological efficacy and molecular mechanisms of MDL-800 using LPS or TGF-β-induced inflammatory and fibrotic models in RAW264.7 macrophages and LL29/DHLF fibroblasts, respectively, as well as a bleomycininduced pulmonary fibrosis model in mice.

    Article Title: NARI-29: A Novel Epalrestat Derivative Attenuates Pulmonary Fibrosis by Modulating the TGF-β/Smad Signaling
    Article Snippet: Pulmonary fibrosis is a chronic and debilitating lung disease marked by excessive collagen and extracellular matrix accumulation, leading to lung scarring and impaired lung function.. Though pirfenidone and nintedanib are approved drugs, their efficacy is suboptimal.. The current study aims to examine the antifibrotic activity of a novel epalrestat (aldose reductase) analog (NARI-29) using in vitro and in vivo rat models.

    Article Title: Hydronidone Mitigates Pulmonary Fibrosis by Regulating the TGF-β/Smad2/3 Axis in <i>in Vitro</i> and <i>in Vivo</i> Models
    Article Snippet: Pulmonary fibrosis (PF) is a progressive, lifethreatening lung disease marked by excessive accumulation of extracellular matrix (ECM), especially collagen, which leads to lung stiffening and impaired respiratory function.. Although antifibrotic drugs like nintedanib and pirfenidone can slow disease progression, their clinical benefits remain modest.. Hydronidone, a structural analogue of pirfenidone, has shown reduced/no hepatotoxicity, improved tolerability (in humans), and proven antifibrotic effects in liver fibrosis.

    Article Title: The tumor microenvironment shows a hierarchy of cell-cell interactions dominated by fibroblasts
    Article Snippet: .. Cells were left for 7 days in culture to assure confluence before performing collagen content measurement using a commercial Sirius Red collagen staining kit (Chondrex, Cat.9046), and measured by Cytation 5-Imaging Reader (Biotek). ..

    Incubation:

    Article Title: Photoprotective Effects of a Hyperoside-Enriched Fraction Prepared from Houttuynia cordata Thunb. on Ultraviolet B-Induced Skin Aging in Human Fibroblasts through the MAPK Signaling Pathway
    Article Snippet: .. After 48 h of incubation, the cultured medium was kept and the collagen content was determined using a Sirius Red collagen staining kit (Chondrex Inc., Redmond, WA, USA). ..

    Cell Culture:

    Article Title: Photoprotective Effects of a Hyperoside-Enriched Fraction Prepared from Houttuynia cordata Thunb. on Ultraviolet B-Induced Skin Aging in Human Fibroblasts through the MAPK Signaling Pathway
    Article Snippet: .. After 48 h of incubation, the cultured medium was kept and the collagen content was determined using a Sirius Red collagen staining kit (Chondrex Inc., Redmond, WA, USA). ..



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    TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using <t>Sirius</t> <t>Red/Fast</t> Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.
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    TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using <t>Sirius</t> <t>Red/Fast</t> Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.
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    TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using <t>Sirius</t> <t>Red/Fast</t> Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.
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    Image Search Results


    TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using Sirius Red/Fast Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.

    Journal: The Journals of Gerontology Series A: Biological Sciences and Medical Sciences

    Article Title: Time-restricted feeding improves metabolic flexibility, promotes beiging, and mitigates fibro-inflammation in the adipose tissue of aged mice

    doi: 10.1093/gerona/glag085

    Figure Lengend Snippet: TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using Sirius Red/Fast Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.

    Article Snippet: The semiquantitative collagen detection kit for total tissue (Sirius Red/Fast Green collagen staining kit, Chondrex, Inc., catalog # 9046, Woodinville, WA) was used to assess the total amount of collagen and noncollagenous proteins in adipose tissue sections from the three experimental groups.

    Techniques: Staining